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KMID : 0351619720130020233
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Kyungpook Medical Journal
1972 Volume.13 No. 2 p.233 ~ p.244
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Ultrastructural Changes of Adrenal Cortex of Rats Substituting 2% Sodium Chloride and Distilled Water in Lieu of Drinking Water
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Abstract
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It is woll known teat various de;rees and combinations of i~e~ressive changes and hemorrhagic disorders of different organs may be observ;:d in sodium ch1_oride intoxication. Re-ports on the uitrastructura} changes of adrenal cortex in sodium c´_nloride intoxication are rare, however.
An attempt was made to study alterations of fine structures of the adrenal cortex by light and electron microscopy.
Twenty albino rats, maintained on a stcc´~ diet, weighing from 1G0 to 310 gm, were divide into 3 groups.
Group 1. Control group.
2 rats: tap water was given as dirnking water. Total daily NaCI intake, MaCl intake,2.2
Group 2.
9 rats: a 2 o NaCl solution was substituted for drinking water. Total daily NaCl intakc, 25.0 mEq.
Group 3.
9 rats: distilled water tivas substituted for drinking water. Total daily NaCI intake, 1.0 mEq.
Three rats were sacrificed at the end of the 1st, and and 3rd experimental weeks from groups 3 and 3. The control group was sacrificed similarly. Adrenal glands were extirpated and studied by both light and electron microscopic examinations.
The results were as follows:
1. Considerable weight less was observed in the group of rats given the 3 % 1\7aC1 solution (group 2), whereas retarded weight increase was noted in the experimental group of rats given distilled water (group 3).
2. In the zona glcmerulosa of the adrenal cortex, a marked atrophic change for the group of rats on the 3o NaCI solution (_group ~) was noted and a mild hydropic s~~ cuing of the cytoplasms of the cells of the zona glomerulosa on the group of rats on the distiiled water (group 3) was observed.
3. Electron microscopic study revealed ghata degenerative change occurred on the group of rats on the 2% NaCl solution (group 2), characterized by an atrophy of sER and the Golgi complex; an increased number of lipid droplets, lysosome and glycogen particles; also a swelling of the mitochondria and a decreased number of mitochondrial cristae; plus a chondriosphere formation. On the other hand, the group of rats on the distiiled water (group 3), had a proliferative change such as an increased number of sER, with the dilatation cf Golgi complex and both a decreased number of lipid droplets and glycogen particles.
It can be concluded, therefore:
The functional activity of the intracellular organelle of the zona glomerulesa of the adrenal glands responsible for synthesis and secretion of mineralccorticoid was depressed when the experimental rats ~~: ere given 3~´, NaCl solution as a substitute for drinking water after a considerable length of time. This functional activity was accelerated when the rats were given di-stilled water in lieu of drinking water over long duration.
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